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The release of inducible nitric oxide synthase (iNOS)-derived NO contributes to the regulation of a variety of physiological and pathophysiological processes. In this study, we evaluated for the first time the constitutive and inducible nitric oxide synthase (cNOS and iNOS) expression in primary human peritoneal mesothelial cells (HPMCs) collected from 13 different regions around the world: Australia (1 region), France (3 regions), Germany (5 regions), Iceland (1 region), Italy (4 regions), Japan (1 region), South Korea (1 region), the Netherlands (1 region), Poland (1 region), Spain (2 regions), Sweden (1 region), Turkey (1 region), and the United States (3 regions). We investigated the age of donors, their race, and sex, time between collection of the cells and RNA extraction, and their passage number. Northern and Western blot analyses showed a significant regional variation in iNOS expression. The lowest levels of iNOS expression were found in Australia, followed by Iceland, the Netherlands, and finally Sweden. In addition, an increasing correlation was found between the abundance of iNOS and the passage number of the mesothelial cells. Exposure to interleukin-1beta increased the iNOS expression in mesothelial cells. The intramesothelial NO production may play a role in the local defense against infectious and/or inflammatory agents or in pathophysiological conditions.
759 So.2d 258 (2000)
STATE of Louisiana, Appellee,
v.
Christopher W. HITCHCOCK, Appellant.
No. 34,692-KA.
Court of Appeal of Louisiana, Second Circuit.
September 27, 2000.
Writ Granted March 13, 2001.
*259 Indigent Defender Board, by Mark H
1a679d06d6

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